Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSDEASRFLAE--LGIADDSVTPITGGWASWTFATG----SGRILRVARNPEIDAAHRRESRLLPELAEAVSFAVPAPTHFGVHKGMTYMVYPRLPGRGLEPG---------------DRVRAVAPMLAELHAFPVDRAAELLGCAVTAEAWRDDYLEIGKWVEGEVLPVLDGELRDRVRREYDATLP--DLAAISPALIHRDLGCEHILTDPETGMPTGIIDFETATVGDPAIDYVGLLVTFGEQVTRELIAASGS---EIS-WRLVRFYHWLGAA--HAVKYGLAERDDAIVADGIEGLRRRLPG 5UXD Chain:A ((6-299)) -DLDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST----SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGA----EF--EIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDE--RITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAYAEGGGRPWPGLARHCTEMFSAAPLGYGLYALATGEAAHREAAAAALNPPEE-

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1194 -19766 -16.55 -74.59 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -16.55 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: