Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQTLSYDEETRGKTVINHMGGVFLYNRPEQILEQYELEVKSISRGRDSYQ----CDTEQGPKILREYRGSKERAGFLADMLDHLSGQGRTVETV--MRTKEGEPFSVNEEETKY-------ILYQAFPGAECDTKNRADMLSAVRELALLHQSAQNYE----GS------VPEFLKSGQNNLLLL--YEKRNRELNKVRN------YIRAKKKKNDF----EMMFAVWYPEYVKKAQETTDI----------LKDLGIQEQLIGFCHGDYNQHNVIFSREGIAVVHFENFLYQESVGDLANFIRKMME----------------KNNWNAGLGMD---LIRGYDRVRKLSPEELKYL------YVYLAYPEKFWKIANRYYNSHKAWLSGRNIEKLEKVVAQEDAREQFLQMLFHFTV 4N0N Chain:A ((22-422)) SAVCTVCGAA----PVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRSTYCTMCEGSPKQMVPKVPHPILDHLLCHIDYGSKEELTLVVADGRTTSPPGRYKVGHKVVAVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAASEYVEGPPGSGKTFHLVKDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGNITVRLPQ-VGTSEGETFVDEVAYFSPVDLARILTQGRVKGY--GDLNQLGCVGPASVPRNLWLRHFVSLEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRGLGHRTIDSIQGCTFPVVTLRLPTP------QSLTRPRAVVA---VTRASQELYIYDPFDQLSGLLKF---

General information: TITO was launched using: RESULT: Template: 4N0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 -966 -0.57 -2.92 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -0.57 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_4N0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n0n-query.scw PDB file : Tito_Scwrl_4N0N.pdb: