Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDQQQIIAVAEAVLTQRYGGQQHLTEPEEL-SGASGTVVLRLRVANNPFLSYRSVVVKY-SPQTDDPIDDAAFLREVVAYQFTTSLSEEARP-GPVMLGYDLEQHAIILTDSGDGDTLATLLENS----DEEQRIRLLRNLGTSLGKMHAGTAGAELSFNTLLARMVRARDGAREIQAMRESMLENRILDGLEIVRRAGIDVPADVADIAGTVQVHMLHGESRAFTPFDLSPDNIIFADRTQFLDYEWAGFRDVIFDVAGVIAGFPQY-ISARPISEAETKVFLEAWIAEVAGVWP-ALQNTETLHSRITAALVGWAFFSVSLLYLDGEEPRGTTRYGGGWIMADNGNYELVQDPEALEEDVERGASVLRSPAHTDF-TAVEKAIRRDIGDAFSALAWYAAAGDTPRNAVIADFASHVAERLK- 1UWH Chain:A ((1-276)) --DDWEI-------------PDGQITVGQRIGSGSFG-TVYKGKWHGD-------VAVKMLNVTAPTPQQLQAFKNEVGVL------RKTRHVNILLFMGYSTAPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHA--------------------------------------------------------------------KS----IIHRDLKSNNIFLHED-------------------------LTVKIGDFGLATVLSGS--ILWMA------PEVIRMQD--KNPYSFQSDVYAFGIVLYELMTGQLP-----YSN------------INNRDQIIFMVGRG---YLSPDLSKVRSNCPKAMKRLMAEC-----LKKKRDERPLFPQILASIELLARSLPK

General information: TITO was launched using: RESULT: Template: 1UWH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1242 553 0.45 2.19 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 0.45 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_1UWH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1uwh-query.scw PDB file : Tito_Scwrl_1UWH.pdb: