Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLSVLFEQISPYLPPQEALKVENIEDGYSNHVMKLHWY--NAPRAVLRVPMLDTEAFLIDRHAELNALKAAQNAGISPTVIWSNDQGAILSEYIHAPALSWDVKHNSRDIKRIGALFTQVHALSVNAPSR---------------NIYQVIEHYVGGIEHHD--TN---G--------AHKHEVAYLQHCFDQMNKVDSRGRPDVLCHNDLNPKNVLLNN-EEIWFIDWEYTALGDPLFDLAAVSRSH---------NLTLDQQKILIEAYDDTLNV---QDTLKTIHDYGFAYALREMAWLLLKHVISVDDHQSLDYYREFKDTKKLNPFI 2QG7 Chain:D ((111-449)) --------------------LEFQIINGG-TNILIKVKDMSKQ-AKYLIRLYGPKTD----NREREKKISCILYNKNIAKKIYVFFT-NGRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCK---RENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHS-----S-DFDFINYGMTRLTAS-

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 985 921 0.94 3.63 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : 0.94 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: