Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRTGDPTDAEVREIGILAGNQRATCDDRAYFQAAAERLAMLPDASEPDVPVLLLEQHYGQQG-A-LTALSSEVERTFDARLADGRAFILKTSSRPQ-ALESFQFQSAALAGLRGADGVMAPKVIDTLSGGLMFEHEGGCGYLQTRMDGIPLHMVPRTPEILREVGAALARLNLAMKDTDPPAARRPVLWNIACWP-WLVELE---RYLSKGRTAKLVRSAMAEYIRDISP-HIADLDWQVTHNDPSPFNMIDTGKGIGFIDFGDGGWNPRVQDLAIAAGHFVIDPRTPLGGAEHVIAGYASLAPLSELEASLLLGLMRARQSALVLINNWRSHLFPAAAPYIMKNVKRAEQGLAVLALLDVSSGEAAIRAAAGLDPP 1ZYL Chain:A ((8-277)) -------------------------------------------FQTLHPDTIMDALFE-HGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVND-EVPVAAPVAF-NGQTLLNHQGFYFAVFPSVGGRQFEAD--NIDQMEAVGRYLGRMHQTGRKQLFIHR---PTIGLNEYLIEPRKLFEDATLIP-SGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKA---EQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYY-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1158 -21122 -18.24 -80.62 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -18.24 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: