Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASAARPIRDYLPGLLVLAGGLYTVYYYYMYWIRVYGALCLPGYGDPVKVEVTQGIWIRLDAILFPTVFGVILALLGVMLVWKKKFGAAVVALVLSATVINWPAFIMIAGGALLGLLFWRKTLLPTIDLPDETMEWVDAEAGLSVLKSFRRLPSERLAYSFHNEGGGRFVVKETSSA---ESAERETRVLNAAAEN---GLPAPEAKATD---GR-FLLMPQLDGFPFLQP-------LDFEAWTDEIAGILTTTHSAPKPDMKAR-RR----PAEPV---IPDRAFD-----RANWERAARIFREE-TADAVPVFVHGDFEPANVLFSG-GRATGLTGWDHAGSGPAQLDVGRMRVALELIHGGNVADRFLAKYKNLSKDRSFRYSRYWDLLAVYGWLEQDPGSIASHWRRFGITTLSEDEVMRRLERFVKTLTDKE 5IQI Chain:C ((11-257)) --------------------------------------------------------------------------------------------------------------------------------NVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN--NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 800 8838 11.05 41.10 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.53 3D Compatibility (PKB) : 11.05 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: