Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPALVLDPECRSDQQIASDFLTYLQSESSDRVAYELEPMRLRGGCDARLYRYKLIDEEPKVLRVLRPE-REVEELMHHQLVHRTLNQQGLKTPLIHRVCGDK--TVLGGVFAVMDLLPGQIVFALDPERHGKVLGESMAAMHGLDVRPIVESLRRAGVPDERFLS--PVFQHKVLGFF-EQTTPW----SADLMAWLRDHLPLDGETLAVIHGDYHGGNVMVDNGSISGVLDWGF-CIADPAVDLAHTMNVYRVLVRQVDPAMSSQVCEQIIDGVLKTYQSIRPLNHERIKACRVFHLLSLLAAGVAGVGPEFVRKPESQRDYLSFIEQTTGIRLSPSA 1ZYL Chain:A ((6-282)) ---------FTFQTLHPDTIMDALFEHGI--RVD-SGLTPL-NSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEAD-NIDQMEAVGRYLGRMHQTGRKQLFI-HRPTI-GLNEYLIEPRKLFEDA-TLIPSGLKAAFLKATDELIAAVTAHWREDF-TVLRLHGDCHAGNILWRD--GPMFVDLDDARNGPAVQDLWMLLNGDKAE------------QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLM------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1176 -58291 -49.57 -219.14 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -49.57 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: