Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNEKIENIFDKFEINIKEIKSATNSFNSNVYIITSVDDKRYV-LKFCNNEKKMLNESKYMKYLKPYIPVADVVGTGDCDEKYYIIQSFFEGSNKFDEEANELSDEQ---VRNIGILLAKLHSCEL--LDESSNSWIIY-LNGCLEKTVETLEKIFGKENNKKISDFLSNYINEKLSNN-YKNSILHMDFRIGNVMFGKN----DEIGLIDLESMKNGEYVFDFVKV---NRLFNNDKFLKF-MEGYNTIKAV-DSNFEERLNFYSLFDS-YTSLWWCATKNQLDSEFYKLNYKIVMKYLAKLNKDGVI------------------------ 3CSV Chain:A ((4-333)) SREDEIRDFLATHGY------ADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDL--GDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMV---FVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1266 -14364 -11.35 -51.30 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -11.35 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: