Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFQLDKDWRLQPI-KGATGQTYMGIKETEKVFIKRNTSP-----------LLAALSKE-GL-APKMIWTKRTANGDVLTAQEWLEGRLLHADE---------IGKRNDVIDVLYQLHHSNLLKDMLKKIGGEVCTPLTMLKEYKRQLPKEL-K-----RNSYLKEVITYLYNHLPEYPETSYVAVHGDVNHRNWLVSN----NYL-YLVDWDSVMVADPAVDLGMILSHYVPRSGWNQWLLSYGLI-PNESTVQRIYWYGLFSFLQEIVRHHQEGERRAMTAEILQLKRMFSI 3TDW Chain:A ((22-297)) --------QSVESLGEGFRNYAILV---NGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAI--SAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHP-VYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGE--LFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRARDQDWISEGLELLEEDK--

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1044 3357 3.22 13.93 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 3.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: