Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDATDELATLIARGLGVLRPKRLHAGAEAVAYELDDARVLKVYHAGGSAERFATIASFYARLNSDR-AGFAVPRILTHGVEGGRAYSVEKRLLGEPLSTRA-H--FLT-DTDLIDAYLAAALRVGRIQVEPEWNSWTMFGEQRHGDWHQHVRGQIESQSRALARMLQPAYWERLANATADVRQHFDR--PFLGKVCLIHGDFHPGNVLVGATGRVLSLVDFGAFTTFGDAAFDVATACGYFS-MYEPDQATTRRSLVARLLRMEGSPPGALVAMYLKLAAFLTCTAYPDDRVAVQDTGHFQWAMSVLFDPMLDELVDRVPH 5IQI Chain:C ((17-284)) ------YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT-I------DNKQNVLEEYILLRE-TIYNDLTD-I---EKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGD-SGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYG-NI--DIEKAKEYQDIVEEYYPIETIVYGIK--------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1142 -33898 -29.68 -130.38 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -29.68 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: