Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNSYAHLNHYISPSAEAGLSDAWRRSLGRLGDYSAILIRSLNLRPLEVSLHDQTEEHILVRIR---LPGSYLILRVAPEDDLAAYVYQIRTLNGHNIPAPQIIQRDLSRSLVPFAFTLESYVPGVTAIEL-QSAPLLRGAGRQAGRALRRMHRIPVTSAGRPTA---SGRWTQQNWRTVLR--QIGRRLAAPPADALIFGEREQ--RVVADLLDHPVLECQQPSLIHGRFSPRAVRCTSSGNVHVEALIDPGHYVGGDGLYDLACGLCAA---------------YPEPWREGLLDGYQSA--GPLNEAERRRLPL----------LQLLTNYWEACRLYMHAEP-HEAARSEA----IRLMEEQVGLTRLVSV------ 4OCV Chain:A ((24-378)) TNEALFDVASHF-----------ALEGTVDSIEPYGDGHINTTYLVTTD------GPRYILQRMNTGIFPDTVNLMR-----NVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSI----PLRVTHNDTKLNNILMDATTG-KARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1436 -27177 -18.93 -89.40 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -18.93 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: