Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLAE-GRLIFISHGENTTFRHIGADGTSHLVRVHRPSRHGRGVDSAAAVDSELAWLRALREDTDLLVPEPLAARDGASAVAVTEAGETRLCSVLRWMDGRIHEASPRPVHLHRLGAAMASLHDQADAWTPPDGFTRIHWDHEAFFGNV-MVYGRTPAAECWT-LLPPELRARFREVGARLADLIPGARDSGLIHADLHLGNALFRRGAVKLIDFDDCGTGPRSYELAVALWELRGRPDYPAFRQALLSGYRARRDIDAT---HLDDFIALRQVAFDLWYTGMAQVNPAFSVRLDGVHQWSLDVLDLVEAG 1ZYL Chain:A ((12-284)) ----------HPDTIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWT-----ADQILEEHQFALQLVND-EVPVAAPVAF-NGQTLLN----HQGFYFAVFPSVGGRQFEAD-NIDQMEAVGRYLGRMHQTGRKQLFI---HRPTIGLNEYLIEPRKLFEDA---TLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKA--EQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRR----------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1199 -51174 -42.68 -191.66 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -42.68 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: