Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFSKELNLIDSLTTSDVEEYIRYFFPESRVISFKIIPEGYENILIKVKCENAFYILRISSMKKNHNMSSYSEKLIKRELDFIKYLQKN-GVPVPTFYKSVADGKTYVKTESKSHVRFITLMNYIKGVHPEYTKDNLQEIASKQVIFHNISIQFQTDYIEND----EEYSAMSHRMISPSKLRTEQ---FPTRLYETMKEI-----YLDVRFELERYYKNTNKQMIHSDIKRDNIFFNQGKLVAFIDFGDIRYSVIAEDLGCFIWDLCDRVYDENGDFAHLVKKYLDDY-----QKYNQNFKK-------------EDQRMAINYAIDRYSIINLHYLVENQGDNERLKYQVNKASKQLEIVKQLLDLRDTQFPYEKKASSC------------ 2OLC Chain:A ((5-396)) -----KTPLYETLNESSAVALAVKLGLT---LTCQEIGDGNLNYVFHIY--DRALIIKQAVPYWP-----LTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIE-GENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAETLIHGDLHTGSIFASEHE-TKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDGADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLD-TIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKLLVK

General information: TITO was launched using: RESULT: Template: 2OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1701 -31182 -18.33 -93.64 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -18.33 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_2OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2olc-query.scw PDB file : Tito_Scwrl_2OLC.pdb: