Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSHLVDVVRRAHPDVDLEGAGV-HSGQFHDVLIA----R-DRVFRFPKTAGAAAELPGRVAVLTAVDAVELGVGVPVPLSEVRDGGPHG----FLVLSRLHGTPLERGDATSPEVIDVVAAEFARVLRAMAGADV-EKLRLVLPVADAGRWRGFAGRVRATLFPLMSEDGRARAERELAAAVA--MDHVATGLVHGDLGGENVLWQQVEELPRLTGIVDWDEAKVGDPAEDLAAVGASYGPELVERVVALLGA------GDLWPRIRAYQGTFALQQALAGAEDGDDEELEDGLTAYR 5IGH Chain:A ((8-294)) -DTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVV--PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVV-NDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDN---TERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAK----

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1189 -13541 -11.39 -50.53 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -11.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: