Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------RLCTVQIMRDDYVHPVAYKKIEFPGPTTKPFQFKW---EIRGVTYNVQVL-YEKCAAKIYQEPALPDGLIIKGH------------------------------------------------------------------------------------------------------------------------ 5J1B Chain:A ((12-275)) NDGVSETLLAWRHIDFWTSEHNPDLNATLSDPCT----QNDITHAEEDLEVSFPNPVKASFKIHDGQEDTSGLFYGFQLMTLDQVVAMTQAWRNVAKNLNKRSPDQKSIPPNAVQPVYAHPAWIPLITDNAGNHIGVDLAPGPNGKYAQIITFGRDFDTKFVIAENWGEFLLSFANDLEAGNWYLVDGELVFRDKKSNGPIQDYFEVLKRRTWIKYQLERPHRD

General information: TITO was launched using: RESULT: Template: 5J1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 159 7834 49.27 118.69 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 49.27 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_5J1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5j1b-query.scw PDB file : Tito_Scwrl_5J1B.pdb: