Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDSQIQSLPEAIRKYIEGREYTIDDMGKSGSKVLIF----EDMVLKITDK-----PCDDKDAVEMMRWLEGKIPAPQVIVFEEDDSYSYLLMTKVRGKVACDKYYLER-PKELVPLLSKSIKMLQSIDITDCPVVKNVDRELKKAAYRVKNGLVDISDAEPDTFGENGRFRDPEELLSWLQENRPPYEPVLSHGDLCLPNILIEKGNISGFIDMGNCGIADKWEDIAILYRSLRHNF-DGTYGKIYPGLEPDSFFEELGIEPDREKIDYYILLDELF 2B0Q Chain:A ((1-263)) ---AKMRISPE-LKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNN---DLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVEL--------FFDLLGIKPDWEKIKYYILLDELF

General information: TITO was launched using: RESULT: Template: 2B0Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1170 56812 48.56 225.44 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 48.56 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_2B0Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2b0q-query.scw PDB file : Tito_Scwrl_2B0Q.pdb: