Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVMRSPLLLAALADAAVPGIRPIKVEGLPVPAGDL---YQSAHITGADGRVWLVRSALTGAAGAELAASDALVQLLTRRVDFDVP-----RVEGSARPKEGSAVAVYARPRGRALDWRHLAGRSAAARALGQALAHLHGVDPRVVEEAGLATYDADTYRQRLRADVDRAAATSRVPSALIARWREALDTPALWRFSTTVTHGPLRGADVHVDEGE-VSAISGWEHAGVADPARDFALLWNSAPQQAFDTVFEAYAAERAERPDPNLERRVRLMGEMELAYALLASRTMGDDARVERHAAALEELAFEVEGDSSLLPPARRAGTAPIDLVPPLDPADIEVVDAEVGEEDATVEIPVRLAPEREDRSEHAGATRVDGDAARTRLPHGSSEEPAASSDDSAGAVADESSGGNARSR 5IGR Chain:A ((21-233)) -----------------------LKLHG-PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 771 5443 7.06 26.68 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.06 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_5IGR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igr-query.scw PDB file : Tito_Scwrl_5IGR.pdb: