Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELEPLLDSLAVLAGQEREVTLLPGGLTNANYRV----TTAT-----RDVVVRVASASSGLLSIDRDQEVHNSRAA----------AKAGVAPEVVEHRPGLLVVQFVP------DALTYGD--SDLAANLAGVADVVRRLHEGPA-FAGRFDMFDIQREYLAIMHTHRFRMPRGYLALAP----AAERMEAALRVHPEPRVPCHNDLLAANFL-------QDAQRLWIVDYEYSGMNEASFELGNIVQE------------------AHLTPEHLAELVTAYV-----------GHEDPVRTARAAL----WGIASAWAWTLWGTIQAGASDLDFDFWEWAMDKMDRAAQAFAEPGFDELLDTVGSPR 4DA5 Chain:A ((110-446)) ----------------EFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLY-----------------AEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKY--LKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFD----------------------

General information: TITO was launched using: RESULT: Template: 4DA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 806 -3347 -4.15 -13.94 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -4.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_4DA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4da5-query.scw PDB file : Tito_Scwrl_4DA5.pdb: