Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFKNNIALGKSVTEALEILERELGSQYCYCKTLEGCSNEVHIIKDVRSGLPYIM-RFTR-QSRFQNYSLEREILSHVVGRGVATSQAYLF--ADGIVAACIEGH-CVESDKMLGDSPYYELIAKQMRRLHEISVQDDGTSVMYSETHYGLKSMLDISVDYIGKGREAELLYKLYSEDGVLGQLVNDHPS-LLWTCISHNDLHSGNIIYSPSAQEVRFIDWEYSTYSINAFDIACFFLEFTGIDCEISAFPCASKRQDFYLYYFGSSSPPIDFLCLFFIPLAC---LFWAAWSSGID--GLD--VYTKNRTRLGHATLRKLVNEIWPQCGLTLDKEDCELLELVDFTFQSLYTN 4R7B Chain:A ((29-304)) -----------------ISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTN--KQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTS--I-KTKFDKIAPILQTIHTSAKELRGE----FAPFEEIKKYESLIEEQIPY----ANYESV---RNAVFSLEKRLADLGVDRKSCHIDLVPENFIESP-QGRLYLIDWEYSSMNDPMWDLAALFLESE---------FTSQEEETFLSHYESDQTP---VSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRAVKGLASYG--------------------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1217 19759 16.24 75.13 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 16.24 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: