Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRTGDPTDAEVREIGILAGNQRATCDDRAYFQAAAERLAMLPDASEPDVPVLLLEQHYGQQGA-LTALSSEVERTFD-ARLADGRAFILKTSSRPQALESFQFQSAALAGLRGADGVMAPKVIDTLSGGLMFEHEGGCGYLQTRMDGIPLHMVP-----RTPEILREVGAALARLNLAMKDTDPPAARRP-VLWNIACWPWLVELERYLSKGRTAKLVRSAMAEYIRDISPHIADLDWQVTHNDPSPFNMIDT-G----KGIGFIDFGDGGWNPRVQDLAIAAGHFVIDPRTPLGGAEHVIAGYASLAPLSELEASLLLG-LMRARQSALVLINNWRSHLFPAAAPYIMKNVKRAEQGLAVLALLDVSSGEAAIRAAAGLDPP 3CSV Chain:A ((4-307)) ----------------------------------------------SREDEIRDFLAT-HGYADWNRTP-------RYQRLRSPTGAKAVLMDWSPE-EGGDTQPFVDLAQYLRNL-DISAPEIYAEEH--------ARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFS---EYRYAILGDAAEDNRKRFEHRFAQIL-SAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRD--VPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFG--KRHYIEFVPRVWAHFERGLAHP-----------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1283 -19381 -15.11 -68.73 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -15.11 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: