Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERLGPLYVKAGQVLSTREDLVPPAVARELESLHDRAAPCPFDRMSAVLEAELGPDWRRRFRHFDTERPLGCASIAQAYAAVLD-DGRSVVVKVQRPGIAAQMTTDMRLLRGMTRTLARRTPVLSETIDFEAMLAVIFEAVRNELDLTLEAANMDEARAAVTAVPGIDVPGVVHATER-------VLIQRRAPGTSVRDADPAAFDDAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCPGGPTTLIDWGMIGRL----------------------DRHLSSTLLMTLLGLANN--------DAHAVARAWSEMGRPTPWA--DIGAFEQDMSVVVPRLASIPLDRLRFGASLATLLRLSTRRGIRTNPMIGLLGKSFANMEGTVRHLAPH--LSATDVLV-RQTGRVLGEYAAQSLSQQQLGFTALQLLSGLETLLPHARAVGRSLSGGDLTLQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 1PMN Chain:A ((8-363)) --------------------------------------------------------------------------------------------DNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRPFQN---------------------------------QTHAKRAYRELVLMKC----------VNHKNIISLLNVFTPQKTLEEFQDVYLVMELMD-ANLCQVIQ-MELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLASFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKL----FPDSLFPADSEHNKLKASQARDLLSKML-------VIDPAKRISVDDALQHPYINVWYDPAEVEAPQLDEREHTIEEWKELIYKEVMN----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1PMN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1387 -10362 -7.47 -34.42 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -7.47 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_1PMN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1pmn-query.scw PDB file : Tito_Scwrl_1PMN.pdb: