Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPAPTDSIYAYLTAAPASGFAGLSVEILEHWRGDENLLWRVRAG--SEEAVVKLFLDAGQAR--GRRQFDGQQLFAATGIAPEPLWFDRYPEGLARQVLVYRWVEGRALQPD---DADDLTALVASLAAVHGGDVATVRRI--------------SPRALNLEYFWSMLSGGLK---P---TQQWLEKSGYPQMAAFLRELEARGQTLVAEGLPLWHAAPPTPIHGDPRPENVVIQA--GRAVLLDWELFGLGDPALEMAELLHQQR-------MRWPGALQEHLLAEYASLSG-D--AAVVARVQLYRKLLPLRDAAFLVSGAQQVSPTERMQPEYDQALPMLIETSTRALMESAEALDDRLVSDAANLRDEVEHLFTSHQNS 2QG7 Chain:E ((110-448)) ---------------------SLEFQII--NG-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFT-----N--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWN-TIWKYFHLLNEERKKICSFDAKANILKLIDFDV---LRDSIVEVES----LCKR--ENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-----DFDFINYGMTRLTA-------------------------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1038 6035 5.81 23.03 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain E : 0.62 3D Compatibility (PKB) : 5.81 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: