Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERLGPLYVKAGQVLSTREDLVPPAVARELESLHDRAAPCPFDRMSAVLEAELGPDWRRR--FRHFDTERPLGCASIAQAYAAVLDD-GRSVVVKVQRPGIAAQMTTDMRLLRGMTRTLARRTPVLSETIDFEAMLAVIFEAVRNELDLTLEAANMDEARAAVTAVPGIDVPGVVHATER----VLIQRRAPGTSVRDADPAAFD-DAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCPGGPTTLIDWGMIGRLDRHLSSTLLMTLLGLANNDAHAVARAWSEMGRPTPWADIGAFEQDMSVVVPRLASIPLDRLRFGASLATLLRLSTRRGIRTN---PMIGLLGKSFANMEGTVRHLAPHLSATDVLVRQTGRVLGEYAAQSLSQQQLGFTALQLLSGLETLLPHARAV-GRSLSGGDLT-----------------------LQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 1APM Chain:E ((10-350)) ---------------------------------------------------------------------------SEQESVKEF-----LAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKIL---------------------------------DKQKVVK------------LKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFAEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKG------------------------------RTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAA------GYPPFFADQPIQIYEKIVSGK---VRFPSHFS--SDLKDLLRNLLQVD--LTKRFGNLKNGVNDIKNH-----KWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF----------------------------

General information: TITO was launched using: RESULT: Template: 1APM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1536 -15453 -10.06 -50.50 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain E : 0.59 3D Compatibility (PKB) : -10.06 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_1APM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1apm-query.scw PDB file : Tito_Scwrl_1APM.pdb: