Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMPQANIIPNDWWRWSDSQPVIIKPLLGGLTNLNYLISVNH---------ELFVLRKNSAISEALNLNRSAEAKALSRADEAGLCAPLIYYDDQHQYMVSRYLGDKTWSVSI--D-HNLSLLAELLRGIHQLPGIDADL--NVENKISCYWQAIDAQA--A---FTR---EL-ISLDSAVGAHITSAKALNNGHVLCHNDLLASNLIISNK------DKLYAIDWEYAAMSDPFYELAVIIEGNAL------------------NAKQQQSLLTRYLR-QPI-------------SALDWQRLYHWQIIYGYLCVLWYAVQYSNGAM-PNIVSEIHRQILAIKALAAKDA 5FTG Chain:A ((30-375)) -------------------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRK--

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 918 -2633 -2.87 -10.17 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -2.87 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: