Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDATDELATLIARGLGVLRPKRLHAGAEAVAYELDD---ARVLKVYHAGGSAERFATIASFYARLNSDRAGFAVPRILTHGVEGGRAYSVEKRLLGEPLSTRAHFLTDTDLIDAYLAAALRVGRIQVEPEWNSWTMFGEQR-HGDWHQHVRGQIESQSRALARMLQPAYWERLANATADVRQHFDRP-F-LGKVCLIHGDFHPGNVLVGAT-GRVLSLVDFGAFTTFGDAAFDVATACGYFS-----MYEPDQATTRRSLVARLLRMEGSPPGALVAMYLKLAAFLTCTAYPDDRVAVQDTGHFQWAMSVLFDPMLDELVDRVPH 4H05 Chain:B ((1-265)) MDDALRALRGR-YPGCEW--VVVEDGASGAGVYRLRGGGRELFVKVAALGA-GVGLLGEAERLVWLAEV--GIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLDVAVALAGLARSLHALDWER---CPFDRSLAVTVPQAARAVAEG----SVDLEDLD-EERK---GWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGR-VGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYG-RGWDGAVS-EEKLAFYRLLDE-----------------------------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1172 -28965 -24.71 -114.48 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -24.71 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: