Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQIVEDAGSLDVFRWPSSSPPSPENLEHAARLCQGALGSTSVAVVATPYNHAGWSAQDCIKVSFPSENRSFIAKVPRLVDENSATACKNEAQRASWASQ-HQIG-PAVIAIDDL---S----GAFAMEFLSGQTLSAQM--MLQDHLKTCTIGLLRRIHDAPSQHYMQ-------IYNAPAAVKRMLQAADKERLLDGDDGLLLESLITWTFQKVGCQPTFQTLVPCHNDFHSQNIMLDQQ-GTLWAIDFEDCDLGDPMWDLGYLTANMGLEPLD-LAGIY-GCNME---Q-KKRLEVFYYLAIGHCAVWSAIHGPLWTQHYRDCMLRLRRGWLRL 5IGH Chain:A ((8-295)) -------------------------DTSQLYALAAR-HGLKLH-GPLTVNE-LGLD-YRIVIATVD-DGRRWVLRIPRRAEVS--AKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVR-RE-----FVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGN---EEYLAAAKA--------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1145 -55273 -48.27 -210.16 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -48.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: