Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPAKWRETIDPFELDYHHFRLTEILGYPHAGNDVFHAKGIYQQQETEVYIKVARQQGA-DIKREIDTILKLNLELAPEIIDY-DQ--NREQFVVTIARQGERL-SMLLNQNKDEKSIDYLFEYGQTLAILHQTQGNFENVKDR---RFF---HIPLE--EHFRE------L-RLEEVYHYLVKHKPERETRCFCHGDFHYANILWKEK--HISAILDFELSGMGNREFDIAWALIVRP-GQRFLLTEEEIREFLRGYASEGT--FQEDLVRYYMVLIYSFFYKMGETEYQDFVRSFLQQNIR 4H05 Chain:B ((26-265)) -------------------------------GAGVYRLR----GGGRELFVKVAALGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPR----EQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDE------------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 951 9614 10.11 44.72 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 10.11 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: