Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLA--EGRLIFISH-GENTTFRHIGADGTSHLVRVHRPSRHGRGVDSAAAV-DSELAWLRALREDTDLLVPEPLAARDGASAVAVTEAGETRLCSVLRWMDG-RIHEASPRPVHLHRLGAAMASLHDQADAWTPPDGF-TRIH---WDHEAFFGNVMVYGRT-PAA-ECWTLLPPELRARFREVGARLADLIPGARDSGLIHADLHLGNALFRR--G-AVKLIDFDDCGTGPRSYELAVALWELRGR-----------PDYPAFRQALLSGYRA--RRDIDAT---HLDDFIALRQVAFDLWYTGMAQVNPAFSVRLDGVHQWSLDVLDLVEAG 4OCV Chain:A ((24-319)) ----------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTD-GPRYILQRMNTGIFP----DTVNLMRNVELVTSTLKAQ-GKETLDIVRTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTP-HRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLL---------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1099 -7633 -6.95 -28.70 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -6.95 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: