Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGRALLNDTWAVVTTPRPIVDAVVR-SVCGRAVERLVPLAGGGLNETYRAEFAGDVAVVVRIARRPT---PWFIDEAHLMAQAAA-AGVPTAQVLGLEHLDHD--------GEPLSFSIQQFLPGRSLEELSGELPAFALERVVAEAGEILARVHSVVPDDQRG--IQHELRLPD--EHEVARVARIVATALGPA--AAAVVERGANFLRQEV--TTRTAPPISLVHGDFCPKNLLIHDG---TVAGFIDWEFAGPASPAYDFAQ-WEVDAGE-PLHDRVDLVRRGYARVADPGTADAGWTPAFAVDWSLEKLGWTNPAAPAQFRRCVDVIARYTVC 6EF6 Chain:A ((27-320)) -----------------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDG-RRVVTVDPAD-VPRHVVHFEMVGGAEPDEES--LT----LDDFQTLGRITASLHEHSQRWTRPAGFGR-FSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGS-------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1199 6425 5.36 24.24 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 5.36 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: