Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLSVLFEQISP-YLPPQE----ALKVENIEDGYSNHVMKLHWYNA---PRAVLRVPMLDTEAFLIDRHAELNALKAAQNAGISPTVIWSNDQGAILSEYIHAPALSWDVKHNSRDIKRIGALFTQVHALSVNAPS----RN----IYQVIEHYVGGIEHHD----T-NGAHKHEVAYLQHCFDQ------------MNK-VDSRGRPDVLCHNDLNPKNVLLNNEEIWFIDWEYTALGDPLFDLAAVSRSHNL------------------TLDQQKILIEAYDDTLNV----------QDTLKTIHDYGFAYALREMAWLLLKHVISVDDHQSLDYYREFKDTKKLNPFI 3MES Chain:A ((50-402)) --DTEIIIGICRKNIPGWKEINESYIEVKQIFSGLTNQLFVVSIVNELKHPRILFRIYGKHV--KFYDSKVELDVFRYLSNINIAPNIIADFP-EGRIEEFIDGEPLTTKQLQLTHICVEVAKNMGSLHIINSKRADFPSRFDKEPILFKRIYLWREEAKIQVSKNNIDKELYSKILEEIDQLEELIMGGEKFSMERALELKLYSPAFSLVFAHNDLQENNLLQTQNNIRMIDYEYSAINFAGADIANYFCEYIYDYCSEKQPYFKFKYEDYPCEELRKLFISVYLSQTLQEQVMPSQQIVHIMTKAVEVFTLISHITWGLWSIA-----------VEFDFTEYANTRFTHY-

General information: TITO was launched using: RESULT: Template: 3MES.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1031 -20472 -19.86 -74.71 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -19.86 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_3MES.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3mes-query.scw PDB file : Tito_Scwrl_3MES.pdb: