Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNCVTSLTASDLPVAEVSHSVLHPEFIAAEVARRYPVKGELTCFLLYRGMNDVYLVQDEETKYALRVWRKTYRDVDDVAYELDFLDYLRQQG-FPASVGVPQHDGKLYFKVVSPEGERAIALYDWAPGVKFGDCLSEETAFKIG-AAMARMHLLGDAWAGPDHTFSTETAKDYNICMPALID--------------------FVYDRPDDLRDYPVIAANLDK-RLDELAASGKVPLGVCHRDFHPSNVHVAQDGAITLLDFDAAGEDFLMQDVQN-FVWGNLFYGFDPKIGEAF-EDGYQSVRPFTKEETEN-IEL-FLMAKALRLIAGMAHSSTAVGRGTLRFRNLDWLGDYVKTRARACGLL 2Q83 Chain:A ((11-346)) ---------------LSAEDAKKLTELAENVLQGWDVQAE----KIDVIQALVWKVHTDSGAVCLKRIH---RPEKKALFSIFAQDYLAKKGMNVPGI-LPNKKGSLYSKH----GSFLFVVYDWIEGRPFE--LTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPYELYDVIREKYVRKSALPKEELESAFEYERIKANALRQLI----

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1284 -18181 -14.16 -59.61 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -14.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: