Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFKNNIALGKSVTEALEILERELG--------SQYCYCKTLEGCSNEVHIIKDVRS-------GLPYIMR-FTRQSRFQNYSLEREILSHVVGRGVATSQAYLFADGIVAACIEGHCVESDKMLGDSPYYELIAKQMRRLHEISVQDDGTSVMYSETHYGLKSMLDISVDYIGKGRE-AELLYK-LY-SEDGVLGQLVNDHP-SLLWTCISHNDLHSGNIIYSPS-----AQEVRFIDWEYSTYSINAFDIACFFLEFTGIDC---------EISAFPCASKRQDFYLYYFGSSSP------------PIDFLCLFFIPLAC---LFWAAWSSGIDG---L--D--VYTKNRTRLGHATLRKLVNEIWPQCGLTLDKEDCELLELVDFTFQSLYTN 5FTG Chain:A ((10-376)) --------------RRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSL-PDISAEIAEKMATFHGMKMPFNKEP---KWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKL---------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1077 -21756 -20.20 -76.07 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -20.20 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: