Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLAEGRLIFISHG-ENTTFR--HIGADGTSHLVRVHRPSRHGRGVDSAAAVDSELAWLRALREDTDLL-VPEPLAARDGASAVAVTEAGETRLCSVLRWMDG-RIHEASPRPVHLHRLGAAMASLHDQADAWTPPDG----FTRIHWDH--EAFFGNVM------VYGRTPAAECWTLLPPELRARFREVGARLADLIPGARDSGLIHADLHLGNALFRRG-AVKLIDFDDCGTGPRSYELAVALWELRGRPDYPAFRQALLSGYRARRDIDATHLDDFIALRQVAFDLWYTGMAQVNPAFSVRLDGVHQWSLDVLDLVEAG 3I1A Chain:B ((8-289)) ----------AQQLIELLKVHYGIDIHTAQFIQGGADTNAFAYQADSE-SKSYFIKLKYGYH--------D--EINLSIIRLLHDS-GIKEIIFPIHTLEAKLFQQ----LKHFKIIAYPFIHAPNGFTQNLTGKQWKQLGKVLRQIHETSVPISIQQQLRKEIYSPKWREIVRSFYNQIEFDNSDDKLTAAFK-SFFNQNSAAIHRLVDTSEKLSKKIQPDLDKYVLCHSDIHAGNVLVGNEESIYIIDWDEPMLAPKERDLMFIGGGVGNVWNKPHEIQYFYEGYGEINV-DKTILSYYRHERIVEDI----------------------------------

General information: TITO was launched using: RESULT: Template: 3I1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1035 -8297 -8.02 -31.43 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -8.02 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_3I1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3i1a-query.scw PDB file : Tito_Scwrl_3I1A.pdb: