Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGIVDDEALDASLRAWIEATTGSG-ITAIQRVSGGAFRTSCRVDLDGAPGAAFLKIDLGSAPRTPFDLRREHELLTRLDGR--ARAPRTIGWHEGA-------TAMAMHCLPGEARWAGIADEAHRDRIERSFAEALVECHAVDIASLALDHLPPGLTIGEAIRRELDMWEELLDANV---ADPDPLTLFAFRWLRHRLPADDRPAVLVQGDAGPGNFLFDA-ERVTGLVDWEMTHLGHPLEDLGCVLARSLVQPMASAERLLSLYRAASGRDWT--MDELLYATILVMARFSVPIALALESRHTGMDFGLTNGYFRLSQISLIRLIARAEGLTLDETPPASGPRPAIGFEFDYLRDVLGTIVRPAIDDPYVQYRLDGAIGLIGYLRAALADDTAGPRGDRDELRALYADRIAEGDIATTLQEFLAEALWRETLMKDMLGPLHGGRVKL 5IGI Chain:A ((6-281)) -------TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVD-DGRRWVLRIPRRAEV--SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLI-----RTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSW-PDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG---EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA---------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1150 -28785 -25.03 -111.14 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -25.03 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: