Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPAPPAVGQRTAWVDVPGDVRRGIEDVLGAPVIHAESQSGGFSPGTADRVR-TSTGRRAFVKAVSPDQ--NPDSPEIHRREADYLAVLPASSRLPKLIGRYDD----GYWIAMVMEEIDGECPPVPWSGAQVETAMETLAALAADLTPNPLPGLEPISEGFSPLFAGWRRLKDDPPTDLDPWIAARLDDLIELSESTLPRIDGDTVVHCDVRADNLLVRADGSMVVVDWPWA-----------LSG-----ADWLDRFMLLINIDLYG-----GHDVEDLVARHLTH--VDPDLITGTLAGLCAFFVDSARQPPAPGLPTLREFQRAQGDSTLAWLRRRLTGDRGV 1O6Y Chain:A ((23-298)) --TPSHLS---------DRYE--LGEILGF---------GGM--SEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAAL-NHPAIVAVYDTGEAETPAGPLPYIVMEYVDG----------------VTLRDIVHTE--GPMT---------------PKRAI--------EVIADACQALNFSHQ------NG--IIHRDVKPANIMISATNAVKVMDFGIARAIAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKN----PENR-YQ-TAAEMRADLVRVHN------

General information: TITO was launched using: RESULT: Template: 1O6Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1124 -21083 -18.76 -91.67 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -18.76 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_1O6Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1o6y-query.scw PDB file : Tito_Scwrl_1O6Y.pdb: