Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSDEASRFLAELGIADDSVTPITGGWASWTFA-TG----SGRILRVARNPEIDAAHRRESRLLPELAEAVSFAVPAPTHFGVHKGMTYMVYPRLPGRGLEPGD---------RVRAVAPMLAELHAFPVDRAAELLGCAVTAEAWRDDYLEIGKWVEGEVLPVLDGELRDRVRREYDATLPDLAAISPALIHRDLGCEHILTDPET--GMPTGIIDFETATVGDPAIDYVGLLVTF----GEQVTRELIAASGS------EISWRLVRFYHWLGAAHAVKYGLAERDDAIVADGIEGLRRRLPG 3CSV Chain:A ((6-277)) --EDEIRDFLATHGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEE-GGDTQPFVDLAQYLRN-LDISAPEIYAEEH--ARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLD-P----ETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIF----------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1000 6877 6.88 28.89 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 6.88 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: