Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPDRPEAEIEAMSALFARHLPGLAARSVTRLGEGMDHVAYEAD---G-GLILRMSKEPDPARRADRIRREAALLALVAEI-SPLPVPRVILSDPDAGVLGYVKLPGRPLAERPV------------AEPGRLAPALGEFLTRLHLAPPERFSALAAPD---TQPLTAWLAEAERHHRAVAAHLPAAARRRIEAFLGGAPPAEPRALAFCHNDLGAEHVLAE-GGTVTGVIDWSDAEIADPAYDLGLLYRDLGPEVTASILAHYAAPWD---DAAGRRAVFYARCALLEDLAYGLRTGPRRYAEAALAHLDRTFSAWG 5UXA Chain:A ((4-299)) --------DIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVM---PRTKVEKQALDLVNKYAISFQAPNWIIYT--EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKF-G-VGENLWNRWQAWLNDD-DMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAKEALEV------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1197 -5468 -4.57 -20.10 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -4.57 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: