Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLSVLFEQISP-YLP-PQ----EALKVENIEDGYSNHVMKLHWYN--------APRAVLRVPMLDTEAFLIDRHAELNALKAAQNAGISPTVIWSNDQGAILSEYIHAPALSWDVKHNSRDIKRIGALFTQVHALSVNAPSR--NIYQVIEHYVGGIEHHDT-N-GAHKH----EVAYLQHCFDQMNK-VDSRGRPDVLCHNDLNPKNVLLNN-------EEIWFIDWEYTALGDPLFDLAAVSRSHNL------------------TLDQQKILIEAYDDTLN--------------VQDTLKTIHDYGFAYALREMAWLLLKHVISVDDHQSLDYYREFKDTKKLNPFI 5FTG Chain:A ((9-369)) ---RRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLGPKLYGIFP-QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS-----IEFGYMDYAQARFDAY-

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1051 16669 15.86 60.83 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 15.86 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: