Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPAPTDSIYAYLTAAPASGF-----AGLSVEILEHWRGDENLLWRVRAGS---------EEAVVKLFLDAGQAR-GRRQFDGQQLFAATGIAPEPLWFDRYPEGLARQVLVYRWVEGRALQPD---DADDLTALVASLAAVHGGDVATVRRIS-PRALNLEYFWSMLSGGLK-PTQQWLEKSGYPQMAAFLRELEARGQTLVAEGLPLWHAAPPTPIHGDPRPENVVIQA-------GRAVLLDWELFGLGDPALEMAELLHQQRM----------------RWPGALQEHLLAEYASLSG--------------DAAVVARVQLYRKLLPLRDAAFLVSGAQQVSPTERMQPEYDQALPMLIETSTRALMESAEALDDRLVSDAANLRDEVEHLFTSHQNS 5FTG Chain:A ((11-369)) ------RAYLWCKEFLPGAWRGLREDEFHISVI--RGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----EAMVLESVMFAILAERSLGPKLYGIFP-------QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFG-TMEKYLKEVLRIKFTEESRIKKLH--KL---LSYNLPLELENLRSLLES--TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS----IEFGYMDYAQARFDAY------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1060 -9005 -8.49 -32.51 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -8.49 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: