Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIRHPTRDTDILRLLQMLGIEASRIVEYRHGIAARNFHIVCMDGTTRVVRYDTRRTAADIREDRMLGT------LAVRNGINAPTGN-WLIHELGNATVVIRQHLAGTTLAELPPATWPDARRLGEVLRSIHEFPQPVIARKFFYAPVLDMLDRQWQTIRDAVASIAETA---DLAALLRYALQQFDHATE----------IETLR-LSPTGLIHGDFTPANILIHEHGLTVL-DWEKSCIGPVCADIAQSLYYFTA-----------HSSK-----IGIFATSFLEG----YGKPDWYKPDVIKAWMT----LHPAFILLTDAANTFINQRLPLPARNP-HR-------------EAYFRDVS---------VPRYRAYLAHQRDLLSIVS 1VZO Chain:A ((31-352)) ------------------------QLLTVKHELRTANL----TGHAEKV-------GIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRT------------ERQVLEHIRQSPFLV---TLHYA---------FQT-ETKLHLILDYINGGE----LFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYDFCGTIEYMAPDIVRGGDDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQE--MSALAKDLIQRLL---MKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDV-----

General information: TITO was launched using: RESULT: Template: 1VZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1162 -22406 -19.28 -89.62 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -19.28 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_1VZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1vzo-query.scw PDB file : Tito_Scwrl_1VZO.pdb: