Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDLDKPIAYGRTAEIFAWG---ENQVLKLFHAWV-GFDAVEYEYRIARTMQSCGLPVPQVGEIIEYKGRYGITCQRIKGI-SMTESLARAPWKILRYARRMAELHARVHAVTHP--TDIP--PLNPKMARKILQA-Q--SLP--ARLREKALTRLEKLPEGHDLCHGDFHPDNILLTP-QGEVILDWMDAGTGNPLADLARTSIILKGSIESGQIRHPLLKRLAHLFHRAYLAQYFSLRPGGE-SEYALWLPIIAAARLSENISELEGWLVAQAERGLS 4R7B Chain:A ((43-277)) -----EQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDI---EAGIKVNEYIESAITLDSTS--IK----TKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEF--T-------------SQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVY---------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 910 1536 1.69 7.01 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 1.69 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: