Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMPQANIIPNDWWRWSDSQPVIIKPLLGGLTNLNYLISVNHELFVLRKNSAISEALNLNRSAEAKALSRADE-AGL-CAPLIYYDD------------QHQYMVSRYLGDKTWSVS-ID-HNLSLLAELLRGIHQLPGIDADLN-VEN---KISCYWQAIDA-------QAAF---TRELISLDSAVGAHITSAKALNNGHVLCHNDLLASNLIISNK---DKLYAIDWEYAAMSDPFYELAVIIEGNAL---NAKQQQSLLTRYLR-QPISALDWQRLYHWQIIYGYLCVLWYAVQYSNGAMPNIVSEIHRQILAIKALAAKDA 6EF6 Chain:A ((44-323)) ---------------------ASLRLL-NLSENATYLVEDGEHQSILRVHRQD-YHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTH--------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1007 -57084 -56.69 -237.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -56.69 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: