Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNCVTSLTASDLPVAEVSHSVLHPEFIAAEVARRYPVKGELTCFLLYRG-MNDVYLVQDEET-KYALRVWRKTYRD-VDDVAYELDFLDYLRQQ-GFPASVGVPQHDGKLYFKVVSPEGERAIALYDWAPGVKFGDCLSEETAFKIGAAMARMHLLGDAWAGP-DHT----------FSTETAKDYNI--CMPALIDFVYDRPDDLRDYPVIAANLDKRLDELAASGKVPLGVCHRDFHPSNVHVAQDGAITLLDFDAAGEDFLMQDVQNFVWGNLFYGFDPKIGEAFEDGYQSVRPFTKEETENIELFLMAKALRLIAGMAHSSTAVGRGTLRFRNLDWLGDYVKTRARACGLL 3F7W Chain:A ((2-269)) -----------------------VNSVAARVTELTGREV-AAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGW--------------DDRTLAMEWVDERPP----TPEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADR-GALTPGDVRLVEKVLDALDH-LAGDPEPPARIHGDLWNGNVLWQDD-GAVVIDPA-AHGGHREADLAMLALFG------LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCL-----------------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1263 -10309 -8.16 -41.07 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -8.16 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: