Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKIALQLKATLENITNLRPVGEDFRWYLKMKCGNCGEISDKWQYIRLMDSVALKGGRGSASMVQKCKLCARENSIEILSSTI-KPYNAEDNENFKTIVEFECRGLEPVDFQPQAGFAAEGVESGTAFSDINLQEKDWTDYDEKAQESVGIYEVTHQFVKC 1ZSO Chain:B ((-3-156)) MKNTVVRIKAELENVKRLFC-DDEYLWIFNIRDSTSSLT-RDNIQFRKTDILEIPNSRGTANFMIKWTEYPKYSTINFVNTKNSCSYEEVNNNEWRDFASFECRGIELIDFFPSNNFIVED-TKGKLYYDVNLSDQNWCDYNEEHEMCVGIYNLEYEVN--

General information: TITO was launched using: RESULT: Template: 1ZSO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 773 14784 19.12 95.38 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 19.12 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.75 QMean score : -0.018

(partial model without unconserved sides chains): PDB file : Tito_1ZSO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zso-query.scw PDB file : Tito_Scwrl_1ZSO.pdb: