Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDSQIQSLPEAIRKYIEGREYTIDDMGKSGSKVLIF----EDMVLKITDK-----PCDDKDAVEMMRWLEGKIPAPQVIVFEEDDSYSYLLMTKVRGKVACDKYYLER-PKELVPLLSKSIKMLQSIDITDCPVVKNVDRELKKAAYRVKNGLVDISDAEPDTFGENGRFRDPEELLSWLQENRPPYEPVLSHGDLCLPNILIEKGNISGFIDMGNCGIADKWEDIAILYRSLRHNF-DGTYGKIYPGLEPDSFFEELGIEPDREKIDYYILLDELF 3TM0 Chain:A ((1-263)) ---AKMRISPE-LKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNN---DLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVEL--------FFDLLGIKPDWEKIKYYILLDELF

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1145 53627 46.84 212.80 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 46.84 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.636

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: