Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSKELEIVKKLTNVADENNIVLNEDGWTSRVYIVNKGEIV-LKFLKNKQYREEFEHEINILKLIKDH-KFNVNIPLISSSEEDNTYIVLN--GLMGKSMSAEIVDALTKEEKRKIGTQIGLFLKKLHTVAYQGKSP---NNESEIFEWFQKSFNRRKRTLKKHFNENELVAIE-DLVTTLPQRSQKLGIVQ---VFCHGDLGYNNILLT-DSLEVGVIDFGDAGYNDQSYDFVGLEDD-------DMLDAVILAYDGDEILKAK--VEIRRQLLPLMEMLFLIDKKDKEGVQQCVKRVRINLKMHEIYNG 5IQE Chain:A ((37-298)) ---------------------------GYDSVAYLVNNEYIFKTKFSTN----KGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEY---------ILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEI------------

General information: TITO was launched using: RESULT: Template: 5IQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1038 287 0.28 1.20 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 0.28 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5IQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqe-query.scw PDB file : Tito_Scwrl_5IQE.pdb: