Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVELTDPDLERLRRRLAPAHIEDLRPLTGGASSLTYAGQRLGDRVVVKMAPPGIQPTRNRDVLRQCRIIKALRATPVPVPDVLWADAGDPPEIPPLFVMSWIDGSSLEPLFDLDCACESEDIVAERFRSAAATMAQLHRIQPAA-IGLGSEPI------VGAEAEIDRWCRALE-TVDPTLAAGWIDLGKALRAAAPPPLPPAVVHGDFRLGNLRAVDGRITGVLDWEIWSVGDPRVDVGWFLINCDRQTYQRATPYAGATPSPAELVSIYCAAAGYRLPDLAWFQALACFKSAATWSLIVKHNRRRHRPDANVEAMAQVLPHLLSRACILLD 3W0O Chain:A ((22-229)) --------------------VSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRH------FASAALPIPEVL--DIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQA---LDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDPLYEV----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 774 -38020 -49.12 -190.10 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -49.12 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_3W0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0o-query.scw PDB file : Tito_Scwrl_3W0O.pdb: