Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLESLEENFRFLTLEVANQARATRD-------ILGKPEKELVER-LIARDDYIDNLKTIIENK---CFSRIHAEEGAEQSESNRIRAIHIICVNLERIADHCVNIVRQIKH---FSDPTFLHRYEFHSMFNLIQSGLDMILP-----VQRSQKVAQALTICKIEHELDNQYKERFDQIQDEMRAQHNADVGDLITALFIFRYLERIGDALLNVGEAFIFAFLGEKIKIHQFQALQETLSQAGYEGSLSEIDFESIWGGRSGCRIRRVKNTNPDPSK---EVIFKEGQKEKIQQERDNIDRWSDLFPGIAPHVVGYNEGGNYASMLVEFLPGCTLDEVVLTTEPEQVRNALFVFEQTLVDVWEDTIDPQDHPSPGFIKQLSGRLDKIQQVHPGILTPHKRIGRITIPSLRKRLEQGTALEQAVQAPFTVLLHGDCNLNNVVYDFEEQRIRFIDLYRSRQADYIQDVSVFLVSNFRMPIFEPSLRARLNWTIRHFYHFARGFAAKHGDTTFSFRLALGLCRSFLTSTRFELDTEFAYEMAERGGYLL-DRLLEHGSKHQEEFTFPEAVLYY- 4OV8 Chain:A ((6-472)) --------DTLNDVIQDPTRRNKLINDNNLLKGIIMGRDGPVPSSRELIVRPD---TLRAIINNRATIETTTMEAEFTETLMESN-------YNSASVKVSAPCITANSEYSESSSFKNTETEKSMYTSSRYLFPQGRIDFTTPDSGDVIKLSPQFTSGVQAAL-AKATGTEKREALQNLFQEYGC--------------VFRTKVHIG--------GVLSAHTMETFSRSENETEVKQDVKAGLEGAVKGWG----GGATAGHGNTQGTITTSQNRKLNVKYIVNGGDYTKIQ----NTEEWVAS----------TNQSEHWRVIEVTEVTA--VADLLPQPIRGQVKDLLKPLLGKWVDVEKV---PGLESLPVSVYRPKGAIPAGWFWLGDTADASKAL--LVKPTLPARSGRNPA--------LTSLHQGSGMTEQPFVDLPQYQ--------------------YLSTYFGSFAHDTPPGSTLR-GLR-PDHVLPGRYEMHGDTIST---AVYVTRP--------VDVPFPEDEAFDLKSLVRVKLPGSGNPPKPRSALKKSMVLFD

General information: TITO was launched using: RESULT: Template: 4OV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2248 89817 39.95 203.67 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 39.95 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.152

(partial model without unconserved sides chains): PDB file : Tito_4OV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ov8-query.scw PDB file : Tito_Scwrl_4OV8.pdb: