Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHDIAALLHKAAPRSEIAEVLPRSGGQLATVFEVRRIAAAPLIIK-LYSPEWAWKQAKEIHVYDLLAPHLGTAIPQVVHAEPEGETHAFTVMTMLTGVPLCETTA--PDYQ-VVYKQLGELLTAIHRIPQPAYGYLTDEVLEPLPSN-DAYMRRQFAKKLQEFEELGGDRALHARLTEYVEQHSGLFASSSRP-VLCHNDFHEGNVLVDPDTWQVQ--GIVDVENAIAADPLIDLAKTQYYSIKNDQAKLSGL-LTGYGEL----PSDWVERTAVYRLYHA-LELWDWFRLI------GVIDPLESIAADLVSLLGTSRVAGRPDAVR---------------- 3CSV Chain:A ((4-333)) ---------SREDEIRDFLATHGYADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNL-DISAPEIYAEEHA---RGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSE----MTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQL------EGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1313 -14543 -11.08 -50.85 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -11.08 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: