Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MTIRHPTRDTDILRLLQMLGIEASRIVEYRHGIAARNFHIVCMDGTTRVVR-------YDTRR----------TAADIREDRMLGTLAVRNGINAPTGNWLIHELGNATVVIRQHLA--------------GTT------------------LAELPPATWPDARRLGEVLRSIHEFPQPVIARKFF-----YAPVLDMLDRQWQTIRDAVASIAETADLAALLRYALQQFDHATEIE----TLRLSPTGLIHGDF---TPANILIHEHGLTVLDWEKSCIGPVCADIAQSLYYFTAHSSKIGI----------FATSFLEGY-------GKP---DWYKPDVIKAWMTL-HPAFILLT--DAANTF------INQRLPLPAR---NPHREAYFRDVSV----------------------PRYRAYLAHQRDLL----SIVS----------------- 1QF5 Chain:A ((1-431)) GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAG--HTLVINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELE----DRGIPV-RERLLLSE--ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDL-----FDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMV------VDVSDLLDQARQR-GDFVMFEGAQGTL-LD---IDHGTYPYVTSSNTTA--GGVAT----GSGLGPRY--VDYVLGILKAYSTRVGAGPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLDVLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGLPQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA

General information: TITO was launched using: RESULT: Template: 1QF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1570 7391 4.71 25.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 4.71 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_1QF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1qf5-query.scw PDB file : Tito_Scwrl_1QF5.pdb: